Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/cmice immunized with T Lymphocytes activated by mixed lymphocyte culture.
Suitable for Monitoring of T cells in peripheral blood, Analysis of NK subsets, Study of Tcell activation. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
PBMC: Add 10 ul of Anitbody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at roomtemperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a Flow cytometer or microscope.
限制
仅限研究用
缓冲液
PBS with 0.08 % sodium azide as preservative and 0.2 % protein carrier.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody (undiluted) at 2-8 °C. Do Not Freeze! Shelf life: One year from despatch.
Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5