OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.
交叉反应 (详细)
Species reactivity (tested):Human.
产品特性
Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-bindingtranscription factor 3, Oct-3, Oct-4
纯化方法
Purified
免疫原
BALB/C mice injected with synthetic peptide of human OCT-4 origin.
Suitable for Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/100 in an ABC method. Positive Control: Seminoma. Cellular Localization: Nuclear. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
Staining ProtocolWe suggest an incubation period of 30 minutes at room temperature. However, dependingupon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require
OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1