The antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
交叉反应 (详细)
Species reactivity (tested):Human, Mouse.
产品特性
Synonyms: Desmoyokin, Neuroblast differentiation-associated protein AHNAK
纯化方法
Protein A Affinity Chromatography
免疫原
Bacterially expressed fragment of N-terminal domain of human AHNAK1
AHNAK
适用: 人
ELISA, IF (cc), IF (p), ICC, IHC (fro), IHC (p)
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunohistochemistry on frozen sections. Western blot (Positive control: HeLa human cervix carcinoma cell line). Immunocytochemistry (Permeabilization is required, positive control: HeLa human cervixcarcinoma cell line). Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH ~7.4, 15 mM Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 to -80 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.Synonyms: Desmoyokin, Neuroblast differentiation-associated protein AHNAK