E. Coli 抗体
适用: E. coli
WB, EIA, Neut
宿主: 小鼠
Monoclonal
286-13310
unconjugated
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- 抗原 See all E. Coli products
- E. Coli
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适用
- E. coli
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This E. Coli antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)
- 产品特性
- Synonyms: E. coli SLT-1 alpha and beta
- 纯化方法
- Purified
- 免疫原
- SLT Toxin from E. coli O157: H7
- 克隆位点
- 286-13310
- 亚型
- IgG1
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- 应用备注
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Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157: H7 extracts when used ata 10 μg/mL concentration. Immunoblot: Detected SLT 1 when used at 1 μg/mL concentration. ELISA: Used as capture antibody at 20 μg/mL concentration (100 μL), Immobilizes SLT 1 fordetection by an HRP conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL distilled water
- 浓度
- 1.0 mg/mL (prior to lyophilization)
- 缓冲液
- 10 mM Phosphate, pH 7.4 containing 0.9 % Sodium chloride.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for two weeksor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
- 背景
- Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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