Bovine Coronavirus 抗体
BCoV
适用: Bovine Coronavirus (BCoV)
EIA
宿主: 小鼠
Monoclonal
5A4
unconjugated
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- 抗原 See all Bovine Coronavirus (BCoV) products
- Bovine Coronavirus (BCoV)
- 适用
- Bovine Coronavirus (BCoV)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Bovine Coronavirus antibody is un-conjugated
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应用范围
- Enzyme Immunoassay (EIA)
- 产品特性
- Synonyms: Spike glycoprotein, S glycoprotein
- 纯化方法
- Protein G chromatography
- 纯度
- > 95 % pure (SDS-PAGE)
- 免疫原
- Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/cmice.
- 克隆位点
- 5A4
- 亚型
- IgG1
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- 应用备注
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ELISA. HIT (hemagglutination inhibition titer).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.8mg/mL (OD280nm E0.1%=1.4)
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Bovine Coronavirus (BCoV)
- Abstract
- BCoV 产品
- 物质类
- Virus
- 背景
- Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Synonyms: E2 protein, Peplomer protein, S glycoprotein, Spike glycoprotein
- UniProt
- P25193
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