This antibody recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts.
交叉反应 (详细)
Species reactivity (tested):Human.
产品特性
Synonyms: Aminopeptidase N, ANPEP, APN, PEPN, Alanyl aminopeptidase, Microsomalaminopeptidase, Aminopeptidase M, gp150, Myeloid plasma membrane glycoprotein CD13
纯化方法
Protein G Chromatography.
免疫原
A cell suspension containing osteoclasts from osteoclastomas
Suitable for use in Flow Cytometry and Immunohistochemistry (acetone-fixed frozensections). We recommend using 1 μg to stain 1.0 x 10^6 cells in Flow Cytometric applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1 mg/mL (OD280nm)
缓冲液
0.01 M PBS, pH 7.2 containing 2 mM EDTA, 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: one year from despatch.
CD13 also known as Aminopeptidase N is a zinc dependent exopeptidase which cleaves amino acids from the N-termini of target proteins including neuropeptides and cytokines. CD13 is expressed on all cells of the myeloid lineage as well as epithelial cells of the intestine, placenta, liver and kidney. CD13 is upregulated in a number of tumour types including lung, melanoma, thyroid and hepatocarcinoma and has been shown to correlate with poor prognosis. CD13 is a crucial molecule in tumour angiogenesis. CD13 is also the receptor for viruses from the coronavirus family.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150