CTDSPL 抗体 (Internal Region)
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- 抗原 See all CTDSPL 抗体
- CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This CTDSPL antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)
- 原理
- CTDSPL
- 序列
- QCNVSLKKQR SRS
- 特异性
- This antibody is expected to recognise both isoforms (NP_001008393.1, NP_005799.2).
- 交叉反应
- 犬, 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1, NP_005799.2.
- 亚型
- IgG
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- 应用备注
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Western Blot: Preliminary testing showed a band at approx 28 kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2 μg/mL antibody staining (calculated Mwt of 29.9 kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and vesicles of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))
- 别名
- CTDSPL (CTDSPL 产品)
- 背景
- CTDSPL, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like, C3orf8, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase-like protein, CTDSP-like, NIF-like protein, NLI-interacting factor 1, RB protein serine phosphatase fro
- 基因ID
- 10217
- NCBI登录号
- NP_001008393, NP_005799
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