CTDSPL 抗体 (Internal Region)
-
- 抗原 See all CTDSPL 抗体
- CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))
-
抗原表位
- Internal Region
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This CTDSPL antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)
- 原理
- CTDSPL
- 序列
- QCNVSLKKQR SRS
- 特异性
- This antibody is expected to recognise both isoforms (NP_001008393.1, NP_005799.2).
- 交叉反应
- 犬, 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1, NP_005799.2.
- 亚型
- IgG
-
-
- 应用备注
-
Western Blot: Preliminary testing showed a band at approx 28 kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2 μg/mL antibody staining (calculated Mwt of 29.9 kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the nuclei and vesicles of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))
- 别名
- CTDSPL (CTDSPL 产品)
- 别名
- C3orf8 antibody, HYA22 antibody, PSR1 antibody, RBSP3 antibody, SCP3 antibody, 2810418J22Rik antibody, AI426263 antibody, NFI1 antibody, NIF antibody, XNIF antibody, ctdspl antibody, ctdspl.L antibody, nif antibody, scp3 antibody, CTD small phosphatase like antibody, TIM23 translocase complex subunit Tim50 antibody, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like antibody, CTD small phosphatase like S homeolog antibody, CTDSPL antibody, SJAG_03560 antibody, Ctdspl antibody, ctdspl.S antibody
- 背景
- CTDSPL, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like, C3orf8, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase-like protein, CTDSP-like, NIF-like protein, NLI-interacting factor 1, RB protein serine phosphatase fro
- 基因ID
- 10217
- NCBI登录号
- NP_001008393, NP_005799
-