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Vimentin 抗体 (C-Term)

Name: Vimentin 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Availability: OutOfStock

Verified VIM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin 抗体 (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin 抗体 (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin 抗体 (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin 抗体 (C-Term))

FACS

5 images

产品编号 ABIN2613493

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

15

14

11

9

7

7

4

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
341

133

130

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
195

130

11

6

6

5

1
山羊
Compatible Secondaries
克隆类型
210

143
多克隆
标记
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
285

129

105

99

95

64

46

40

39

33

23

19

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

原理

Vimentin

序列

QVINETSQHH DDLE

交叉反应

Cow, 犬, 人, 小鼠, Pig, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

亚型

IgG

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应用备注

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

说明

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

Vimentin (VIM)

别名

VIM (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

VIM, vimentin, FLJ36605

基因ID

7431, 22352, 81818

NCBI登录号

NP_003371

途径

Caspase Cascade in Apoptosis