The antibody detects endogenous level of ATPase only when phosphorylated at serine 16.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
免疫原
ATPase (phospho Ser16) antibody was raised against a peptide sequence around phosphorylation site of serine 16 (A-V-S (p)-E-Q) derived from Human ATPase.
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.