AQP2 抗体 (C-Term) (APC)
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- 抗原 See all AQP2 抗体
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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抗原表位
- C-Term
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AQP2 antibody is conjugated to APC
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein A Purified
- 免疫原
- Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
- Top Product
- Discover our top product AQP2 Primary Antibody
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- 应用备注
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- WB (1:2000)
- IHC (1:200)
- ICC/IF (1:400)
- optimal dilutions for assays should be determined by the user.
- 说明
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0.5 μg/ml of ABIN2486376 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
- 别名
- Aquaporin 2 (AQP2 产品)
- 别名
- AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody
- 背景
- Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
- 基因ID
- 25386
- NCBI登录号
- NP_037041
- UniProt
- P34080
- 途径
- Response to Water Deprivation
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