Phosphoserine 抗体 (Atto 488)
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- 抗原 See all Phosphoserine products
- Phosphoserine
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phosphoserine antibody is conjugated to Atto 488
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- Phosphoserine conjugated to KLH, and phosvitin mixture
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- 应用备注
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- WB (1:500)
- ICC/IF (1:50)
- ELISA (1:250)
- IP (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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2 μg/ml of ABIN2486251 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 μM okadaic acid.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- Phosphoserine
- Abstract
- Phosphoserine 产品
- 物质类
- Amino Acid
- 背景
- Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
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