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DNMT1 抗体 (AA 637-650) (Biotin)

Name: DNMT1 抗体 (AA 637-650) (Biotin)
Brand: antibodies-online
SKU: ABIN2486005
Availability: OutOfStock

DNMT1 适用: 人 WB, IHC, IP, ChIP 宿主: 小鼠 Monoclonal 60B1220-1 Biotin

Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220. (DNMT1 抗体 (AA 637-650) (Biotin))

Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220. (DNMT1 抗体 (AA 637-650) (Biotin))

IHC

3 images

产品编号 ABIN2486005

发货至: 中国

Datasheet as PDF

抗原 See all DNMT1 抗体

DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
抗原表位
22

21

15

12

12

10

9

8

7

7

7

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 637-650
适用
188

54

45

3

1

1

1

1

1

1

1
人
宿主
152

36

1

1
小鼠
Compatible Secondaries
克隆类型
142

48
单克隆
标记
75

14

13

11

8

8

5

5

5

5

5

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This DNMT1 antibody is conjugated to Biotin
应用范围
113

63

42

37

29

28

19

15

14

12

12

5

4

3

3

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

特异性

Detects ~180 kDa. It will cross-react with mouse DNMT1.

交叉反应

鱼, 人, 小鼠, 斑马鱼

纯化方法

Protein G Purified

免疫原

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

克隆位点

60B1220-1

亚型

IgG1 kappa

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anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-270) antibody
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anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody
DNMT1 适用: 人 WB, IHC, IP, ChIP 宿主: 小鼠 Monoclonal 60B1220-1 unconjugated

anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1317-1616) antibody
DNMT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5G2 unconjugated

anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 22-126) antibody
DNMT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注
- WB (1:1000)
- IHC (1:1000)
- optimal dilutions for assays should be determined by the user.
说明

2 μg/ml of ABIN2486005 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Conjugated antibodies should be stored at 4°C
抗原

DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

别名

DNMT1 (DNMT1 产品)

别名

cb1075 antibody, cb983 antibody, dmnt1 antibody, fb05h01 antibody, mta antibody, wu:fb05h01 antibody, ADCADN antibody, AIM antibody, CXXC9 antibody, DNMT antibody, HSN1E antibody, MCMT antibody, DDM2 antibody, DECREASED DNA METHYLATION 2 antibody, DMT01 antibody, DMT1 antibody, DNA METHYLTRANSFERASE antibody, DNA METHYLTRANSFERASE 01 antibody, DNA METHYLTRANSFERASE 1 antibody, MET2 antibody, METHYLTRANSFERASE 2 antibody, METHYLTRANSFERASE I antibody, METI antibody, methyltransferase 1 antibody, Cxxc9 antibody, Dnmt antibody, Dnmt1o antibody, MTase antibody, Met-1 antibody, Met1 antibody, MommeD2 antibody, m.MmuI antibody, DNA methyltransferase 1 antibody, DNA (cytosine-5-)-methyltransferase 1 antibody, methyltransferase 1 antibody, DNA methyltransferase (cytosine-5) 1 antibody, DNMT1 antibody, dnmt1 antibody, Dnmt1 antibody, MET1 antibody

背景

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

基因ID

1786

NCBI登录号

NP_001370

UniProt

P26358

途径

SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection