TLR4 抗体 (AA 420-435) (PE)
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- 抗原 See all TLR4 抗体
- TLR4 (Toll-Like Receptor 4 (TLR4))
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抗原表位
- AA 420-435
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TLR4 antibody is conjugated to PE
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
- 交叉反应
- 人, 小鼠
- 纯化方法
- Protein A Purified
- 免疫原
- Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
- Top Product
- Discover our top product TLR4 Primary Antibody
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- 应用备注
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- WB (1:500)
- IHC (1:50)
- optimal dilutions for assays should be determined by the user.
- 说明
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2 μg/ml of ABIN2485976 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- TLR4 (Toll-Like Receptor 4 (TLR4))
- 别名
- TLR4 (TLR4 产品)
- 别名
- ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody
- 背景
- The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).
- 基因ID
- 7099
- NCBI登录号
- NP_612564
- UniProt
- O00206
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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