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TCP1 alpha/CCTA 抗体 (C-Term) (PE)

Name: TCP1 alpha/CCTA 抗体 (C-Term) (PE)
Brand: antibodies-online
SKU: ABIN2485908
Availability: OutOfStock

TCP1 适用: 小鼠 WB, IF, IP, ICC 宿主: 大鼠 Monoclonal 23c PE

Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 23c . (TCP1 alpha/CCTA 抗体 (C-Term) (PE))

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 23c . (TCP1 alpha/CCTA 抗体 (C-Term) (PE))

IF/ICC

3 images

产品编号 ABIN2485908

发货至: 中国

Datasheet as PDF

抗原 See all TCP1 alpha/CCTA (TCP1) 抗体

TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
抗原表位
40

18

18

15

15

15

15

9

5

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
172

120

78

22

21

20

19

18

17

15

13

11

10

4

4

3

2

2

2

1

1
小鼠
宿主
153

27

18

1

1
大鼠
Compatible Secondaries
克隆类型
147

53
单克隆
标记
67

14

12

10

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This TCP1 alpha/CCTA antibody is conjugated to PE
应用范围
128

53

52

45

45

41

39

27

23

22

13

11

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

Detects ~60 & ~92 kDa. The addition of an Ala (LDDA COOH) prevents binding in ELISA to immobilized syn peptide. This ab also recog the p102B' COP subunit of Golgi coatomer. Not react with human HSP60.

交叉反应

Cow, 犬, Hamster, 人, 小鼠, 兔, 大鼠, 绵羊

纯化方法

Protein G Purified

免疫原

Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.

克隆位点

23c

亚型

IgG2c

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TCP1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-T-Complex 1 (TCP1) (N-Term) antibody
TCP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

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TCP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

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TCP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-T-Complex 1 (TCP1) (C-Term) antibody
TCP1 适用: 小鼠 WB, IF, IP, FACS, ICC 宿主: 大鼠 Monoclonal 91a unconjugated

anti-T-Complex 1 (TCP1) (AA 600-680) antibody
TCP1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-063R unconjugated Recombinant Antibody

anti-T-Complex 1 (TCP1) (C-Term) antibody (HRP)
TCP1 适用: 小鼠 WB, IF, IP, FACS, ICC 宿主: 大鼠 Monoclonal 91a HRP

应用备注
- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN2485908 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Conjugated antibodies should be stored at 4°C
抗原

TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

别名

TCP1 alpha (TCP1 产品)

别名

CCT-alpha antibody, CCT1 antibody, CCTa antibody, D6S230E antibody, TCP-1-alpha antibody, AI528772 antibody, CCT antibody, Cct1 antibody, Ccta antibody, TRic antibody, Tcp-1 antibody, Tp63 antibody, c-cpn antibody, p63 antibody, TRiC antibody, CCTalpha antibody, BEST:GH05123 antibody, CG5374 antibody, Dmel\\CG5374 antibody, T-cpl antibody, TCP-1alpha antibody, TCPA_DROME antibody, Tcp1 antibody, Tcp1-alpha antibody, gh05123 antibody, cct-alpha antibody, ccta antibody, tcp1 antibody, tcp1-a antibody, tcp1a antibody, tcp1alpha antibody, CHUNP6875 antibody, fa13h08 antibody, wu:fa13h08 antibody, wu:fc95g06 antibody, t-complex 1 antibody, T-complex protein 1 subunit alpha antibody, t-complex protein 1 antibody, Tcp1-like antibody, t-complex 1 S homeolog antibody, TCP1 antibody, cct-1 antibody, Tcp1 antibody, T-cp1 antibody, tcp1.S antibody, tcp1 antibody

背景

T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.

基因ID

21454

NCBI登录号

NP_038714

UniProt

P11983