HSP70 抗体 (FITC)
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- 抗原 See all HSP70 抗体
- HSP70 (Heat Shock Protein 70 (HSP70))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP70 antibody is conjugated to FITC
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Antibody Array (AA), BioImaging (BI)
- 特异性
- Detects ~70 kDa. Does not cross-react with HSC70 (HSP73).
- 交叉反应
- C. elegans, Carp, 小鸡, Cow, 犬, Drosophila melanogaster, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, 大鼠, 绵羊
- 纯化方法
- Protein G Purified
- 免疫原
- Human HSP70
- 克隆位点
- C92F3A-5
- 亚型
- IgG1
- Top Product
- Discover our top product HSP70 Primary Antibody
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anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, ELISA, IHC, IF, FACS, ICC, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated
anti-Heat Shock Protein 70 (HSP70) antibodyHSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 70 (HSP70) antibody (HRP)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Heat Shock Protein 70 (HSP70) antibody (FITC)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-Heat Shock Protein 70 (HSP70) antibody (PE)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal PE
anti-Heat Shock Protein 70 (HSP70) antibody (APC)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal APC
anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal PerCP
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Atto 488
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Atto 594
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- 应用备注
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- WB (1:1000)
- IHC (1:10000)
- ICC/IF (1:1000)
- FACS (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2485869 was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- HSP70 (Heat Shock Protein 70 (HSP70))
- 别名
- HSP70 (HSP70 产品)
- 别名
- APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
- 背景
- HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
- 基因ID
- 3303
- NCBI登录号
- NP_005336
- UniProt
- P0DMV8, P0DMV9
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