HDEL 抗体 (C-Term) (Biotin)
HDEL
适用: Saccharomyces cerevisiae
WB, IF, ICC
宿主: 小鼠
Monoclonal
2E7
Biotin
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- 抗原 See all HDEL products
- HDEL
- 抗原表位
- C-Term
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适用
- Saccharomyces cerevisiae
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HDEL antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~78 kDa.
- 交叉反应
- Drosophila melanogaster, 植物, Saccharomyces cerevisiae
- 纯化方法
- Protein G Purified
- 免疫原
- Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
- 克隆位点
- 2E7
- 亚型
- IgG2b
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2484713 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- HDEL
- 别名
- HDEL (HDEL 产品)
- 背景
- HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
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