Phosphotyrosine 抗体 (Biotin)
Quick Overview for Phosphotyrosine 抗体 (Biotin) (ABIN2484576)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
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纯化方法
- Protein G Purified
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免疫原
- Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
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亚型
- IgG1
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anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated
anti-Phosphotyrosine antibody (Atto 488)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 488
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
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应用备注
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- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2484576 was sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
抗原
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