GIT1 抗体 (AA 375-770) (Biotin)
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- 抗原 See all GIT1 抗体
- GIT1
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抗原表位
- AA 375-770
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GIT1 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~90 kDa. Does not cross-react with GIT2.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
- 克隆位点
- S39B-8
- 亚型
- IgG1
- Top Product
- Discover our top product GIT1 Primary Antibody
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anti-GIT1 (GIT1) (AA 460-640) antibody
KO Validated GIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 470-642) antibodyGIT1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 533-561), (C-Term), (Tyr554) antibodyGIT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB15351 unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibodyGIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibody (HRP)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 HRP
anti-GIT1 (GIT1) (AA 375-770) antibody (FITC)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 FITC
anti-GIT1 (GIT1) (AA 375-770) antibody (PE)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 PE
anti-GIT1 (GIT1) (AA 375-770) antibody (APC)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 APC
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- 应用备注
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2483505 was sufficient for detection of GIT1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- GIT1
- 别名
- GIT1 (GIT1 产品)
- 别名
- Cat-1 antibody, p95Cat antibody, GIT ArfGAP 1 antibody, G protein-coupled receptor kinase interacting ArfGAP 1 antibody, G protein-coupled receptor kinase-interactor 1 antibody, GIT1 antibody, Git1 antibody
- 背景
- G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
- 基因ID
- 83709
- NCBI登录号
- NP_114002
- UniProt
- Q9Z272
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