DNAJB1 抗体 (HRP)
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- 抗原 See all DNAJB1 抗体
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DNAJB1 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~40 kDa. Does not cross-react with HDJ2 or YDJ1.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Recombinant Protein HSP40 (Hdj1)
- 克隆位点
- 3B9-E6
- 亚型
- IgG1
- Top Product
- Discover our top product DNAJB1 Primary Antibody
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anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody
DNAJB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1770 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 2-340) antibodyDNAJB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠, 大鼠, 犬, 猴, Cow, Pig, 豚鼠, Hamster, 鱼, 小鸡, 兔, 绵羊, 非洲爪蟾, Mussel, Scallop, Beluga WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 74-123) antibodyDNAJB1 适用: 人, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: Saccharomyces cerevisiae WB, ELISA, IP 宿主: 小鼠 Monoclonal 2A7-H6 unconjugated
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- 应用备注
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- WB (1:2000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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0.5 μg/ml of ABIN2481899 was sufficient for detection of HSP40 (HDJ1) in 15 μg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
- 别名
- HSP40 (DNAJB1 产品)
- 别名
- HSPF1 antibody, Hdj1 antibody, Hsp40 antibody, RSPH16B antibody, Sis1 antibody, CG10578 antibody, DNAJ-1 antibody, DNAJ1 antibody, DROJ1 antibody, Dmel\\CG10578 antibody, DnaJ1 64EF antibody, DroJ1 antibody, EU3500 antibody, HSP40 antibody, HSP40/HDJ1 antibody, Hsp-40 antibody, anon-WO0140519.166 antibody, anon-WO0172774.135 antibody, dHDJ1 antibody, dHDJ1/HSP40 antibody, dHdj1 antibody, dhdJ1 antibody, dhdj-1 antibody, dhdj1 antibody, dnaJ-1 antibody, droj1 antibody, hsp40 antibody, dnajb4 antibody, hdj1 antibody, hspf1 antibody, sis1 antibody, DnaJ-5 antibody, MAS5 antibody, dnaJ antibody, 0610007I11Rik antibody, dnajb1 antibody, zf-Hsp40 antibody, zgc:101068 antibody, DnaJ heat shock protein family (Hsp40) member B1 antibody, DnaJ-like-1 antibody, DnaJ (Hsp40) homolog 5 antibody, type I HSP40 co-chaperone YDJ1 antibody, DnaJ protein antibody, molecular chaperone DnaJ antibody, DnaJ (Hsp40) homolog, subfamily B, member 1a antibody, DNAJB1 antibody, Dnajb1 antibody, DnaJ-1 antibody, dnajb1 antibody, DnaJ-5 antibody, YDJ1 antibody, dnaJ antibody, dnajb1a antibody
- 背景
- Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
- 基因ID
- 3337
- NCBI登录号
- NP_006136
- UniProt
- P25685
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