HSP70/HSC70 抗体 (Atto 488)
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- 抗原 See all HSP70/HSC70 (HSC70-4) products
- HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))
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适用
- 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP70/HSC70 antibody is conjugated to Atto 488
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Detects ~72 (HSP) and ~73 kDa (HSC).
- 交叉反应
- Beluga, 小鸡, Cow, 犬, Drosophila melanogaster, 鱼, 豚鼠, Hamster, 人, 小鼠, Pig, 兔, 大鼠, Saccharomyces cerevisiae, 绵羊, 非洲爪蟾
- 纯化方法
- Protein G Purified
- 免疫原
- Chicken HSP70/HSP90 complex
- 克隆位点
- BB70
- 亚型
- IgG2a
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anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 unconjugated
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibodyHSC70-4 适用: 人 WB, IP, IHC, FACS, IEM, ICC, IF 宿主: 小鼠 Monoclonal N27F3-4 unconjugated
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (HRP)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 HRP
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (FITC)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 FITC
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Biotin)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Biotin
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (PE)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 PE
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (APC)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 APC
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (PerCP)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 PerCP
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Atto 594)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Atto 594
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Atto 390)HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Atto 390
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- 应用备注
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- WB (1:1000)
- IHC (1:200)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2481614 was sufficient for detection of HSP70 and HSC70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))
- 别名
- HSP70/HSC70 (HSC70-4 产品)
- 别名
- BAP74 antibody, CG4264 antibody, Dmel\\CG4264 antibody, E(csp)1545 antibody, E(nd)195 antibody, HSC-70 antibody, HSC4 antibody, HSC70 antibody, HSC70-4 antibody, Hsc-4 antibody, Hsc4 antibody, Hsc4p antibody, Hsc70 antibody, Hsp-c4 antibody, Hsp70 antibody, anon-WO0118547.237 antibody, bs17d06.y1 antibody, dhsc70 antibody, hsc4 antibody, hsc70 antibody, hsc70-4 antibody, hsp70 antibody, i190 antibody, l(3)03550 antibody, l(3)L3929 antibody, l(3)j7A4 antibody, scd antibody, Heat shock protein cognate 4 antibody, Hsc70-4 antibody
- 背景
- HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
- 基因ID
- 423504
- NCBI登录号
- NP_001006686
- UniProt
- P08106
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