Ubiquitin 抗体 (Atto 390)
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- 抗原 See all Ubiquitin 抗体
- Ubiquitin
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ubiquitin antibody is conjugated to Atto 390
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- 特异性
- Detects ~10 kDa. It also recognizes ubiquinated proteins.
- 交叉反应
- 小鸡, Cow, 犬, Drosophila melanogaster, 鱼, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, Rainbow Trout, 大鼠, Saccharomyces cerevisiae, Salmon, 绵羊, 非洲爪蟾
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- Native bovine Ubiquitin, conjugated to KLH
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 大鼠, 小鼠, Cow, Pig, 兔, 犬, 马 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (APC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC
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- 应用备注
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN2481445 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- Ubiquitin
- 别名
- Ubiquitin (Ubiquitin 产品)
- 别名
- CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
- 背景
- Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
- 基因ID
- 281370
- NCBI登录号
- NP_776558
- UniProt
- P0CG53
- 途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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