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V5 Epitope Tag 抗体

WB, ELISA, IP, IF, IHC (fro), RIA, FACS 宿主: 小鼠 Monoclonal SV5-Pk1 unconjugated
产品编号 ABIN2477047
发货至: 中国
  • 抗原 See all V5 Epitope Tag products
    V5 Epitope Tag
    适用
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    宿主
    • 63
    • 43
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    小鼠
    克隆类型
    • 62
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    单克隆
    标记
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    This V5 Epitope Tag antibody is un-conjugated
    应用范围
    • 90
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Flow Cytometry (FACS)
    原理
    MOUSE ANTI V5-TAG
    产品特性
    Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
    纯化方法
    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    免疫原
    Paramyxovirus Simian-Virus 5 (SV5)
    克隆位点
    SV5-Pk1
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline,0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
  • Hornemann, Richard, Rütti, Wei, von Eckardstein: "Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).

    Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, (2001) (PubMed).

  • 抗原
    V5 Epitope Tag
    别名
    V5-TAG (V5 Epitope Tag 产品)
    物质类
    Tag
    背景
    Synonyms: PK-TAG
    UniProt
    P11207
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