V5 Epitope Tag 抗体
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- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 适用
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This V5 Epitope Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Flow Cytometry (FACS)
- 原理
- MOUSE ANTI V5-TAG
- 产品特性
- Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- 免疫原
- Paramyxovirus Simian-Virus 5 (SV5)
- 克隆位点
- SV5-Pk1
- 亚型
- IgG2a
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline,0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
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Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).
: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, (2001) (PubMed).
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Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).
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- 抗原
- V5 Epitope Tag
- 别名
- V5-TAG (V5 Epitope Tag 产品)
- 物质类
- Tag
- 背景
- Synonyms: PK-TAG
- UniProt
- P11207
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