This antibody is expected to recognise both reported isoforms (NP_001094345.1, NP_689811.2), which are identical.
纯化方法
MARCH10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
MARCH10 antibody was raised against an 11 amino acid synthetic peptide near the internal region of 40978.
ELISA: Antibody detection limit dilution 1:8000. Approximately 90 kDa band observed in rat testis lysates (calculated MW of 90.5 kDa according to human NP_689811.2 and of 87.9 kDa according to mouse NP_766156.2). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
MARCH10
(Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))