Urocortin 3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Urocortin 3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of Urocortin 3.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of approximately. 19 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1- 2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.