Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
VPS37C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
VPS37C antibody was raised against a 15 amino acid synthetic peptide near the internal region of VPS37C.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 38 kDa band observed in human duodenum lysates (calculated MW of 38.7 kDa according to NP_060436.4). Recommended concentration: 0.03 - 0.1 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows strong cytoplasm staining in stromal cells. Recommended concentration, 3-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.