ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
纯化方法
SNX26 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SNX26 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of SNX26.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 75 kDa band in human bone marrow, kidney, and pancreas lysates after 1 μg/mL antibody staining. SNX26 antibody may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4 kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human brain shows cytoplasm staining of Purkinje cells in cerebellum. Recommended concentration, 2.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.