S3-12 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
S3-12 antibody was raised against a 16 amino acid synthetic peptide near the internal region of S3-12.
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 45 kDa and 50 kDa in human adipose lysates after 0.1 μg/mL antibody staining.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human pancreas shows membranous and cytoplasm staining in adipocytes. Recommended concentration, 3-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.