Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody is expected to recognize both reported isoforms (NP_001026879.1and NP_060833.1).
纯化方法
Renalase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Renalase antibody was raised against a 10 amino acid synthetic peptide near the internal region of Renalase (aa 224-233).
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 37 kDa band observed in human kidney and mouse kidney lysates (calculated MW of 37.8 kDa according to NP_001026879.1 and 34.9 kDa according to NP_060833.1). Recommended concentration: 0.1-0.3 μg/mL. IHC: In paraffin embedded Human Kidney shows vesicular staining in the epithelial cells of the CT. Recommended concentration, 2-4 g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.