Parvalbumin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Parvalbumin antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of Parvalbumin.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 12 kDa band observed in human brain lysates (calculated MW of 12.1 kDa according to NP_002845.1). Recommended concentration: 0.1-0.3 μg/mL. Approximately 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfected with human PVALB, but not in untransfected rat heart cells. Data kindly provided by Dr Wang Wang, University of Michigan
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.