This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
纯化方法
ORC3L antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ORC3L antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ORC3L.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3 kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.