Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize both human isoforms of this protein.
纯化方法
MID2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
MID2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of MID2.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in mouse heart lysates (calculated MW of 81.3 kDa according to NP_036348.1 and 77.9 kDa according to NP_438112.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.