ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 35 kDa and 60 kDa bands observed in human brain and human liver lysates at 1 on limit dilution 1:8,000. Western Blot: Approximately 35 kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0 kDa according to NP_064695.2). Recommended concentration: 0.03-0.1 ? of primary. Please note that the band we observe is different from the predicted size of approximately 79 kDa according to NP_005658.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.