This antibody is expected to recognize isoform 1 (NP_009106) only.
纯化方法
ERLIN2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ERLIN2 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of ERLIN2.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 43 kDa band observed in human breast lysates and in lysates of human breast tumor (calculated MW of 37.8 kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13,282 (28) :20104-15., PMID: 17502376). Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.