EDD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
EDD1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of EDD1.
UBR5
适用: 小鼠
ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution >1:32000. Western Blot: Approximately 35-40 kDa band observed in human testis, human muscle, A549 and A431 lysates at 0.5-1 ?of primary, this band was successfully blocked by incubation with the immunizing peptide. Please note that the band we observe is different from the predicted size of approximately 322 kDa according to NP_056986. Immunohistochemistry: In paraffin embedded human testis shows nuclear staining in . Recommended concentration, 1-2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
UBR5
(Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))