Immunohistochemistry (IHC), Western Blotting (WB), ELISA
特异性
This antibody is expected to recognize isoforms 1 and 3 ( NP_872584.1 and NP_079043.2 resp) but not isoform 2 (NP_006085.2).
纯化方法
DLC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DLC1 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of DLC1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 28 kDa band in human liver and lung lysates as well as in different epithelial lung cell lines after 0.3 μg/mL antibody staining.1 and 54.6 kDa according to NP_079043.2 (isotope 3). The 28 kDa band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.