Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
DAZL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DAZL antibody was raised against an 11 amino acid synthetic peptide near the internal region of DAZL.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 35 kDa band observed in human ovary and human testes lysates (calculated MW of 33.2 kDa according to NP_001342.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded mouse testis shows cytoplasm staining of spermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatids but this additional staining is not in agreement with results obtained by another antibody. Recommended concentration, 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.