ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
纯化方法
CREB3L4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CREB3L4 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of CREB3L4.
ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 50 kDa band observed in human placenta lysate (predicted MW of 45 kDa according to NP_570968). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows strong staining of cytoplasm in the secretory cells of the gland. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
CREB3L4
(cAMP Responsive Element Binding Protein 3-Like 4 (CREB3L4))