Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
B5 Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
B5 Receptor antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of B5 Receptor.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 38-40 kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5 kDa according to NP_006351.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)