Western Blotting (WB), ELISA, Immunoprecipitation (IP)
特异性
This antibody is expected to recognize both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.
纯化方法
Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Arrestin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Arrestin.
SAG
适用: 人
WB, ELISA, IHC
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows mainly nuclear staining of Purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
SAG
(S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))