Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
Annexin A10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Annexin A10 antibody was raised against a 15 amino acid synthetic peptide near the N-Terminus of Annexin A10.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: At 1 μg/mL antibody banded at approximately 45-48 kDa in Hela, A431 and human kidney lysates. Immunohistochemistry: In paraffin embedded human pancreas shows staining of islet of Langerhans. Recommended concentration, 3-5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.