Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody is expected to recognize the alpha (ADH1A, NP_ 000658), the beta (ADH1B, NP_000659) and gamma (ADH1C, NP_000660) polypeptide variants of human alcohol dehydrogenase.
纯化方法
ADH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ADH1 antibody was raised against an 11 amino acid synthetic peptide near the N-Terminus of ADH1.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 38 kDa band observed in human liver lysates (calculated MW of 39.9 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 0.3-1.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.