ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 50 kDa band in human brain lysates and 48 kDa in rat brain lysates after 1 μg/mL antibody staining. The observed band was successfully blocked by incubation with the immunizing peptide. In transfected MCF7 transiently expressing human UGT8 a band of approximately. 65 kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5 kDa according to NP_003351.2. Recommended concentration, 1-3 μg/mL. Preliminary experiments gave an approximately 50 kDa band in human -brain lysates and 48 kDa in rat brain lysates after 1μg/mL antibody staining. . The observed band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.