ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 200 kDa band observed in Jurkat lysate (calculated MW of 215 kDa according to NP_000250.3). Recommended concentration: 1-3 μg/mL. Immunofluorescence: Transverse sections of mouse skeletal muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (R der et al, Proc Natl Acad Sci U S A. 2010 Feb 2,107 (5) :2031-6, PMID: 20133847). Recommended concentration: 1-3 3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.