TRIM29 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRIM29 antibody was raised against a 12 amino acid synthetic peptide near the internal region of TRIM29.
ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 70 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 65.8 kDa according to NP_036233.2). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration, 1-2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.