This antibody is expected to recognize isoform 1 (NP_000396.2) only.
纯化方法
GM2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
GM2A antibody was raised against an 11 amino acid synthetic peptide near the internal region of GM2A (aa 179-189).
ELISA: Antibody detection limit dilution 1:4000. Western Blot: In transfected HEK293 transiently expressing full-length human GM2A (myc and DYKDDDDK tagged), a band of approximately 28 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.