This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
纯化方法
DCX antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DCX antibody was raised against an 11 amino acid synthetic peptide near the internal region of DCX (aa 232-242).
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 45 kDa band observed in mouse fetal brain lysates (calculated MW of 49.3 kDa according to human NP_000546.2 and 40.6 kDa according to human NP_835365.1 and mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.