This antibody is expected to recognize isoform a (NP_008830.2) and isoform c (NP_777281.1).
纯化方法
IDH3B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
IDH3B antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of IDH3B (aa 369-383).
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 40 kDa band observed in lysates of mouse fetal kidney and fetal skeletal muscle and of cell line NIH3T3 (calculated MW of 42.2 kDa according to human NP_008830.2 and mouse NP_570954.1). Recommended concentration: 0.03-0.1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.