Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
FOXI3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
FOXI3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of FOXI3.
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 50 kDa band in human skin lysates after 1 μg/mL antibody staining . The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Bronchus shows strong nuclear staining in the basal cells of the resporatory epithelium. Recommended concentration, 5-10μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.