This antibody is expected to recognize all isoforms (NP_742057.1, NP_000494.2, NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1
纯化方法
EYA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
EYA1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of EYA1.
EYA1
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 750
应用备注
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 55 kDa band observed in lysates of cell lline HEK293 (calculated MW of 60.7 kDa according to NP_742056.1). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.