ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
纯化方法
PEBP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PEBP4 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of PEBP4.
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band observed in human heart lysates (calculated MW of 25.7 kDa according to NP_659399.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
PEBP4
(Phosphatidylethanolamine-Binding Protein 4 (PEBP4))